The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 43 kDa.
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
ab119357, at 1/100 dilution, staining ERK1 in untreated and siRNA knockdown treated HeLa cells (Formalin fixed and permeabilized with 0.1% Triton X-100) by Immunoflourescence followed by DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1/400.
Nuclei (blue) were stained with Hoechst 33342 dye.
ab119357, at 1/100 dilution, staining ERK1 in deparaffinized Human colon cancer tissue by Immunohistochemistry using a goat anti-mouse HRP secondary antibody at a dilution of 1/500 and colorimetric detection using metal enhanced DAB.
Note: strong cytoplasmic localization of ERK1.
References for Anti-ERK1 antibody [12D11] - ChIP Grade (ab119357)
has not yet been referenced specifically in any publications.
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