Overview

  • Product name
    Anti-ERK1 antibody [1E5]
    See all ERK1 primary antibodies
  • Description
    Mouse monoclonal [1E5] to ERK1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human ERK1 aa 9-143.
    Database link: P27361

  • Positive control
    • Recombinant ERK1 protein; Hela, Jurkat, RAW264.7, HEK293, K562, NIH3T3, Cos7 and PC12 cell lysates; Human breast cancer and bladder cancer tissues; NIH3T3 cells; Hela cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR118551-12 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab119933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • Mapk3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Images

  • Anti-ERK1 antibody [1E5] (ab119933) at 1/500 dilution + Recombinant ERK1 protein

    Predicted band size : 43 kDa
  • All lanes : Anti-ERK1 antibody [1E5] (ab119933) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : RAW264.7 cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : K562 cell lysate
    Lane 6 : NIH3T3 cell lysate
    Lane 7 : Cos7 cell lysate
    Lane 8 : PC12 cell lysate


    Predicted band size : 43 kDa
  • ab119933, at 1/200 dilution, staining ERK1 in paraffin-embedded Human breast cancer tissue by Immunohistochemistry with DAB staining.
  • ab119933, at 1/200 dilution, staining ERK1 in paraffin-embedded Human bladder cancer tissue by Immunohistochemistry with DAB staining.
  • Immunofluorescence analysis of ERK1 in NIH3T3 cells using ab119933 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Hela cells using ab119933 at 1/200 dilution (blue) and negative control (red).
  • ELISA using ab119933.

References

This product has been referenced in:
  • Luo J  et al. MicroRNA-7 inhibits the malignant phenotypes of non-small cell lung cancer in vitro by targeting Pax6. Mol Med Rep 12:5443-8 (2015). Read more (PubMed: 26135959) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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