Anti-ERK1 antibody [E19] (ab78918)
Key features and details
- Mouse monoclonal [E19] to ERK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ERK1 antibody [E19]
See all ERK1 primary antibodies -
Description
Mouse monoclonal [E19] to ERK1 -
Host species
Mouse -
Specificity
Recognizes only the ERK1 protein (e.g.: from human and mouse cells) -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human ERK1 aa 1-379.
Database link: P27361 -
Positive control
- HEK293 and Jurkat cell lysates.
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General notes
For longer term storage add 0.1% Na Azide.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
E19 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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IP |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
IP
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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Database links
- Entrez Gene: 5595 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- Unigene: 861 Human
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Alternative names
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
Images
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All lanes : Anti-ERK1 antibody [E19] (ab78918) at 1/10000 dilution
Lane 1 : HEK293 cell lysate at 1 µg/ml
Lane 2 : HEK293 cell lysate at 5 µg/ml
Lane 3 : HEK293 cell lysate at 10 µg/ml
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Detection of ERK1 by Western Blot of Immunprecipitate.
ab78918 at 0.5µg, 1µg, and 2µg (lanes 1-3 respectively) immunoprecipitating ERK1 from 50µg Jurkat cell lysate.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab78918 has not yet been referenced specifically in any publications.