Customer reviews and FAQs

Overview

  • Product name
    Anti-ERK1 + ERK2 antibody [9B3]
    See all ERK1 + ERK2 primary antibodies
  • Description
    Mouse monoclonal [9B3] to ERK1 + ERK2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ERK1 + ERK2 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1). SwissProt IDs: P27361 and P28482

  • Positive control
    • HepG2 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab36991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 44 kDa.

Target

  • Function
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p42-MAPK antibody
    • PRKM 2 antibody
    see all

Anti-ERK1 + ERK2 antibody [9B3] images

  • Western blot - ERK1 + ERK2 antibody [9B3] (ab36991)
    Western blot - ERK1 + ERK2 antibody [9B3] (ab36991)
    All lanes : Anti-ERK1 + ERK2 antibody [9B3] (ab36991)

    Lane 1 : U87MG cell lysates
    Lane 2 : HepG2 cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : A431 cell lysates


    Predicted band size : 44 kDa
    Observed band size : 44,45 kDa (why is the actual band size different from the predicted?)

References for Anti-ERK1 + ERK2 antibody [9B3] (ab36991)

This product has been referenced in:
  • Schütz I  et al. Lysosomal Dysfunction Caused by Cellular Accumulation of Silica Nanoparticles. J Biol Chem 291:14170-84 (2016). Read more (PubMed: 27226546) »
  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . Read more (PubMed: 25347857) »

See all 8 Publications for this product

Publishing research using ab36991? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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3 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-ERK1 + ERK2 antibody [9B3]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
3 µg
Specification
Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% gradient gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
Username

Dr. Carl Atkinson

Verified customer

Submitted Dec 13 2011

I would like to test ab4819 for use on termite samples.

DISCOUNT CODE: *** Expiration date: March 30th, 2012 I am very pleased to hear you would like to accept our offer and test ab4819 in termite. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submi...

Read More

Western blot abreview for Anti-ERK1 + ERK2 antibody [9B3]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Dispersed rat cortical neurons)
Loading amount
25 µg
Specification
Dispersed rat cortical neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
abreview image
Username

Dr. Sriharsha Kantamneni

Verified customer

Submitted May 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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