- Datasheet
- Specific References (8)
- Protocols
Overview
- Product nameAnti-ERK1 + ERK2 antibody [9B3]
See all ERK1 + ERK2 primary antibodies - DescriptionMouse monoclonal [9B3] to ERK1 + ERK2
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to Human ERK1 + ERK2 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1). SwissProt IDs: P27361 and P28482
- Positive control
- HepG2 cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride -
Concentration information loading... - PurityIgG fraction
- ClonalityMonoclonal
- Clone number9B3
- IsotypeIgG2b
- Light chain typekappa
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab36991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. Predicted molecular weight: 44 kDa. |
Target
- FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. - Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. - DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
- Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. - Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
see all
Anti-ERK1 + ERK2 antibody [9B3] images
-
![Western blot - ERK1 + ERK2 antibody [9B3] (ab36991)](http://a.abcam.com/ps/datasheet/Images/36/ab36991/ab36991_1.jpg)
Western blot - ERK1 + ERK2 antibody [9B3] (ab36991)All lanes : Anti-ERK1 + ERK2 antibody [9B3] (ab36991)
Lane 1 : U87MG cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : A431 cell lysates
Predicted band size : 44 kDa
Observed band size : 44,45 kDa (why is the actual band size different from the predicted?)
References for Anti-ERK1 + ERK2 antibody [9B3] (ab36991)
This product has been referenced in:
- Schütz I et al. Lysosomal Dysfunction Caused by Cellular Accumulation of Silica Nanoparticles. J Biol Chem 291:14170-84 (2016). Read more (PubMed: 27226546) »
- Shimko MJ et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . Read more (PubMed: 25347857) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"