- Datasheet
- Specific References (10)
- Protocols
Overview
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Product nameAnti-ERK1 + ERK2 antibody [9B3]
See all ERK1 + ERK2 primary antibodies -
DescriptionMouse monoclonal [9B3] to ERK1 + ERK2
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human ERK1 + ERK2 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1). SwissProt IDs: P27361 and P28482
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Positive control
- HepG2 cell lysates.
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab184699 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number9B3
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IsotypeIgG2b
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab36991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. Predicted molecular weight: 44 kDa. |
Target
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FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
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Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
see all
Images
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![Western blot - Anti-ERK1 + ERK2 antibody [9B3] (ab36991)](http://a.abcam.com/ps/products/36/ab36991/Images/ab36991_1.jpg)
Western blot - Anti-ERK1 + ERK2 antibody [9B3] (ab36991)All lanes : Anti-ERK1 + ERK2 antibody [9B3] (ab36991)
Lane 1 : U87MG cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : A431 cell lysates
Predicted band size: 44 kDa
Observed band size: 44,45 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Li F et al. Psoralen stimulates osteoblast proliferation through the activation of nuclear factor-?B-mitogen-activated protein kinase signaling. Exp Ther Med 14:2385-2391 (2017). Read more (PubMed: 28962172) »
- Schütz I et al. Lysosomal Dysfunction Caused by Cellular Accumulation of Silica Nanoparticles. J Biol Chem 291:14170-84 (2016). Read more (PubMed: 27226546) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"