Overview

  • Product nameERK1 + ERK2 peptide

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab108469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-ERK1 + ERK2 antibody - Loading Control (ab94484)

  • Purity70 - 90% by HPLC.

  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • ERK 1
    • ERK 2
    • ERK-2
    • ERK1
    • erk1/2
    • ERK2
    • ERT1
    • ERT2
    • Extracellular signal regulated kinase 1
    • Extracellular signal-regulated kinase 2
    • MAP kinase 1
    • MAP kinase 2
    • MAP kinase isoform p42
    • MAP kinase isoform p44
    • MAPK 1
    • MAPK 2
    • MAPK 3
    • Mapk1
    • MAPK2
    • MAPK3
    • Mitogen-activated protein kinase 1
    • Mitogen-activated protein kinase 2
    • MK01_HUMAN
    • p38
    • p40
    • p41
    • p42-MAPK
    • PRKM 2
    see all
  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt

References for ERK1 + ERK2 peptide (ab108469)

ab108469 has not yet been referenced specifically in any publications.

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