Anti-ERK1 (phospho T202 + Y204) antibody (ab24157)

Overview

  • Product nameAnti-ERK1 (phospho T202 + Y204) antibodySee all ERK1 primary antibodies ...
  • Description
    Rabbit polyclonal to ERK1 (phospho T202 + Y204)
  • Specificityab24157 reacts with Phospho-Thr202/Thr204 ERK/MAPK protein.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human, Xenopus laevis
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding Phospho-Thr202 and Phospho-Tyr204 of ERK/MAPK(Rat).

  • Positive control
    • rat brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab24157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Target information above from: UniProt accession P27361 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK antibody
    • ERK-1 antibody
    • ERK1 antibody
    • ERT 2 antibody
    • ERT2 antibody
    • Extracellular Signal Regulated Kinase 1 antibody
    • Extracellular signal related kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • HGNC6877 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin Stimulated MAP2 Kinase antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 3 antibody
    • MAP Kinase antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 3 antibody
    • MAPK antibody
    • MAPK1 antibody
    • Mapk3 antibody
    • MGC20180 antibody
    • Microtubule Associated Protein 2 Kinase antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen Activated Protein Kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK03_HUMAN antibody
    • MNK1 antibody
    • OTTHUMP00000174538 antibody
    • OTTHUMP00000174541 antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 3 antibody
    • PRKM3 antibody
    • Protein Kinase Mitogen Activated 3 antibody
    see all

Anti-ERK1 (phospho T202 + Y204) antibody images

References for Anti-ERK1 (phospho T202 + Y204) antibody (ab24157)

ab24157 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The concentration of this antibody is 0.1- 0.2 mg/ml. Please contact us again if you have any additional questions.

Thank you for your email. We have not had any complaints regarding this antibody. I would suggest increasing the concentration to 1:500 overnight at 4C. Also, please make sure that the protein transferred and that your secondary antibody is working pro...

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Thank you for your enquiry. Just because there is Erk present in your samples does not mean that there is also dually phosphorylated (or active) Erk present as well. There are two possibilities here: either there is no pErk present in these samples, or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"