Anti-ERK1 (phospho T202) antibody (ab47310)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202) antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 (phospho T202) -
Host species
Rabbit -
Specificity
ab47310 detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human ERK1 aa 150-250 (phospho T202). Swiss-Prot #: P27361/P28482
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Positive control
- Extracts from A431 cells, EGF-treated.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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Notes |
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IHC-P
Use a concentration of 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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Database links
- Entrez Gene: 5595 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 50689 Rat
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: Q63844 Mouse
- SwissProt: P21708 Rat
- Unigene: 861 Human
see all -
Alternative names
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
Images
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All lanes : Anti-ERK1 (phospho T202) antibody (ab47310) at 1/500 dilution
Lane 1 : EGF treated A431 cell extract (200ng/ml,30min)
Lane 2 : A431 cell extracts
Predicted band size: 43 kDa -
ab47310 (4µg/ml) staining ERK1 (Phospho T202) in human colon.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab47310 has been referenced in 2 publications.
- Korolkova OY et al. Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets. PLoS One 15:e0231711 (2020). PubMed: 32298357
- Tor YS et al. Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile. PLoS One 10:e0127441 (2015). WB ; Human . PubMed: 26047480