Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)

Overview

  • Product nameAnti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody
  • Description
    Rabbit polyclonal to ERK1 (phospho Y204) + ERK2 (phospho Y187)
  • SpecificityThis antibody is specific for ERK1, only when phosphorylated at tyrosine 204
  • Tested applicationsIHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived around the phosphorylation site of ERK1 tyrosine 204 and ERK2 tyrosine 187 (T-E-YP-V-A).

  • Positive control
    • Extracts from Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab47339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 44, 42 kDa).
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.

Target

Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody images

  • All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with PMA (200ng/ml, 15min)
    Lane 2 : Extracts from Jurkat cells un-treated


    Predicted band size : 44, 42 kDa
    Observed band size : 42,44 kDa
  • Ab47339 staining human normal or colon tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract treated with PMA


    Predicted band size : 44, 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)

ab47339 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (Human Granulosa Cell Line)
Specification Human Granulosa Cell Line
Permeabilization Yes - Ethanol and Triton
Fixative Paraformaldehyde
Username

Mr. FRANCESCO ELIA MARINO

Verified customer

Submitted Jun 10 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Human Cell lysate - whole cell (Human granulosa cell line)
Specification Human granulosa cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Mr. FRANCESCO ELIA MARINO

Verified customer

Submitted Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"