Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab16869)

Overview

  • Product nameAnti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibodySee all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies ...
  • Description
    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • SpecificityThis antibody recognises ERK1 phosphorylated at Thr202 and Tyr204 and ERK2 phosphorylated at Thr185 and Thr187.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • NIH3T3 cell lysates stimulated with PDGF.

Applications

Our Abpromise guarantee covers the use of ab16869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 43 kDa.

Target

  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p42MAPK antibody
    • p42MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • P44ERK1 antibody
    • P44MAPK antibody
    • PRKM 1 antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody images

  • Western Blot analysis of NIH3T3 cell lysates (10µg) stimulated with PDGF

References for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab16869)

This product has been referenced in:
  • Velten M  et al. Systemic maternal inflammation and neonatal hyperoxia induces remodeling and left ventricular dysfunction in mice. PLoS One 6:e24544 (2011). WB ; Human . Read more (PubMed: 21935422) »

See 1 Publication for this product

Product Wall

Thank you for your patience in this matter.

I am very pleased to inform you that the product (positive control lysate for ab16869 - NIH3T3 cell lysate stimulated with PDGF) is now available and we are now doing the final internal necessary st...

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Thank you for your kind reminder.

I will be sending an e-mail as soon as have further information about the lysate from the Lab.

Thank you for your patience in advance.

I can now inform you that we do not have the lysate in stock currently. This is expected to be available at the earliest next month.

I am sorry for the inconvenience. If you have any further questions or concerns please do not hesitate to con...

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Thank you for your enquiry regarding ab16869.

The cells need to be stimulated and in order to be able to detect phosphorylated ERK1 + ERK2 PDGF is recommended as inducer.

Your customer may wish to take a look at this specific public...

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Application Western blot
Sample Mouse Cell lysate - whole cell (BV2 cell line)
Loading amount 400000 cells
Specification BV2 cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 08 2008

Application Western blot
Sample Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount 20 µg
Specification spinal cord homogenate
Treatment sham or nerve transected
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 08 2008

Application Western blot
Sample Rat Cell lysate - whole cell (HAPI cell line)
Loading amount 400000 cells
Specification HAPI cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10 % SDA-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Primary Microglia)
Loading amount 400000 cells
Specification Primary Microglia
Treatment LPS 50ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Jun 09 2008

Thank you for filling out our technical help questionaire so thoroughly. You protocol looks correct, however I advise for this or any other antibody against a phosphorylated protein that you avoid using milk in your diluents and blocking buffer. Milk c...

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Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"