Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
(Peptide available as
Our Abpromise guarantee covers the use of ab4819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44,42 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 10µl for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Peptide Competition: Extracts prepared from PC12 cells stimulated with NGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50