Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab76165)

Overview

  • Product nameAnti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibodySee all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies ...
  • Description
    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Specificityab76165 may also recognise the unphosphorylated forms of ERK1 + ERK2.
  • Tested applicationsICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Conjugated to an immunogenic carrier protein.

Applications

Our Abpromise guarantee covers the use of ab76165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 44 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P Use a concentration of 10 - 50 µg/ml. Antigen retrieval is not essential but may optimise staining.
IHC-Fr Use a concentration of 10 - 50 µg/ml.

Target

  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERK1 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p42MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody images

  • ab76165 staining ERK1 + ERK2 (phospho T202 + Y204) in U87MG cells treated with zaprinast (ab120940), by ICC/IF. Increase of ERK1 + ERK2 (phospho T202 + Y204) expression correlates with increased concentration of zaprinast, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120940 (zaprinast) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76165 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab76165) at 1/500 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Mouse hippocampus lysate
    Lane 3 : Mouse hippocampus lysate
    Lane 4 : Mouse hippocampus lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 10 minutes
  • All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab76165) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 1 minute

References for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab76165)

This product has been referenced in:
  • Sun D  et al. Sox9-related signaling controls zebrafish juvenile ovary-testis transformation. Cell Death Dis 4:e930 (2013). IHC ; Zebrafish . Read more (PubMed: 24263104) »
  • Weidemann W  et al. Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression. Glycobiology 20:107-17 (2010). Read more (PubMed: 19797319) »

See all 3 Publications for this product

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Attached please find the MSDS you requested. Please do not hesitate to contact me if you have any additional questions.

Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (14% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted May 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"