Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Conjugated to an immunogenic carrier protein.
Our Abpromise guarantee covers the use of ab76165 in the following tested applications.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 44 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|IHC-P||Use a concentration of 10 - 50 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|IHC-Fr||Use a concentration of 10 - 50 µg/ml.|
ab76165 staining ERK1 + ERK2 (phospho T202 + Y204) in U87MG cells treated with zaprinast (ab120940), by ICC/IF. Increase of ERK1 + ERK2 (phospho T202 + Y204) expression correlates with increased concentration of zaprinast, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120940 (zaprinast) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76165 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A anti-rabbit DyLight 488 polyclonal secondary antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.