Erk1 (pT202/pY204) + Erk2 (pT185/pY187) ELISA Kit (ab126444)

Overview

  • Product name
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate
  • Assay type
    Semi-quantitative
  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    ab126444 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human, Mouse and Rat cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187). An anti-pan Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Erk1 (T202/Y204)/Erk2 (T185/Y187) antibody is used to detect phosphorylated Erk1 (T202/Y204)/Erk2 (T185/Y187). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab126444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126444.
  • A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126444.
  • A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed by Western Blot.

Protocols

References

ab126444 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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