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ab126444 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human, Mouse and Rat cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187). An anti-pan Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Erk1 (T202/Y204)/Erk2 (T185/Y187) antibody is used to detect phosphorylated Erk1 (T202/Y204)/Erk2 (T185/Y187). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2x Cell lysate buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187)||2 vials|
|Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody||1 unit|
|HRP-conjugated anti-rabbit IgG||1 x 25µl|
|Positive Control: lyophilized powder from A431 cell lysate||1 vial|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab126444 has not yet been referenced specifically in any publications.