ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187)||1 vial|
|Detection Antibody Erk1/2||1 vial|
|Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody||1 unit|
|HRP-conjugated anti-rabbit IgG: 500x HRP-conjugated anti-rabbit IgG concentrate||1 x 25µl|
|HRP-Streptavidin Concentrate||1 x 200µl|
|Positive Control: lyophilized powder from A431 cell lysate||1 vial|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab126445 has not yet been referenced specifically in any publications.