RabMAb

Anti-ERK1 (pY204) + ERK2 (pY187) antibody [EP197Y] (ab76299)

Overview

  • Product nameAnti-ERK1 (pY204) + ERK2 (pY187) antibody [EP197Y]
  • Description
    Rabbit monoclonal [EP197Y] to ERK1 (pY204) + ERK2 (pY187)
  • SpecificityThis antibody detects Erk1 phosphorylated at Tyrosine 204.
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 204 of human Erk1.

  • Positive control
    • A431 cell lysate treated with EGF
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/20.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    Anti-ERK1 (pY204) + ERK2 (pY187) antibody [EP197Y] images

    • All lanes : Anti-ERK1 (pY204) + ERK2 (pY187) antibody [EP197Y] (ab76299) at 1/2000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : A431 cell lysate treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References for Anti-ERK1 (pY204) + ERK2 (pY187) antibody [EP197Y] (ab76299)

    This product has been referenced in:
    • Jin H  et al. Matrine activates PTEN to induce growth inhibition and apoptosis in V600EBRAF harboring melanoma cells. Int J Mol Sci 14:16040-57 (2013). WB ; Human . Read more (PubMed: 23912239) »
    • Wang Y  et al. Hypoxia-Inducible Factor-1alpha and MAPK Co-Regulate Activation of Hepatic Stellate Cells upon Hypoxia Stimulation. PLoS One 8:e74051 (2013). WB ; Human . Read more (PubMed: 24040163) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"