Validated using a knockout cell line

Anti-ERK5 antibody [EP791Y] (ab40809)


  • Product name
    Anti-ERK5 antibody [EP791Y]
    See all ERK5 primary antibodies
  • Description
    Rabbit monoclonal [EP791Y] to ERK5
  • Host species
  • Tested applications
    Suitable for: WB, ICC, Flow Cyt, IPmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ERK5 aa 800-900 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Hela Cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab40809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 89 kDa).
ICC 1/250 - 1/500.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function
      Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
    • Tissue specificity
      Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
    • Cellular localization
      Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Big MAP kinase 1 antibody
      • BMK 1 antibody
      • BMK 1 kinase antibody
      • BMK-1 antibody
      • BMK1 antibody
      • BMK1 Kinase antibody
      • EC antibody
      • ERK 4 antibody
      • ERK 5 antibody
      • ERK-5 antibody
      • ERK4 antibody
      • ERK5 antibody
      • Extracellular signal regulated kinase 5 antibody
      • Extracellular signal-regulated kinase 5 antibody
      • MAP kinase 7 antibody
      • MAPK 7 antibody
      • MAPK7 antibody
      • Mitogen activated protein kinase 7 antibody
      • Mitogen-activated protein kinase 7 antibody
      • MK07_HUMAN antibody
      • OTTHUMP00000065906 antibody
      • OTTHUMP00000065907 antibody
      • PRKM 7 antibody
      • PRKM7 antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: MAPK7 (ERK5) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab40809 observed at 88 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab40809 was shown to specifically react with ERK5 in wild-type HAP1 cells as signal was lost in MAPK7 (ERK5) knockout cells. Wild-type and MAPK7 (ERK5) knockout samples were subjected to SDS-PAGE. Ab40809 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing A549 cells stained with ab40809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40809, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-ERK5 antibody [EP791Y] (ab40809) at 1/5000 dilution + Hela cell lysate at 10 µg

      Predicted band size: 89 kDa
      Observed band size: 115 kDa (why is the actual band size different from the predicted?)

      The predicted weight of 89 kDa is for the precursor version of human ERK5 protein. However, ab40809 detects endogenous levels of total Erk5 protein which appears around 115 kDa in SDS PAGE


    This product has been referenced in:
    • Li L  et al. Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells. Med Sci Monit 22:2357-62 (2016). WB ; Human . Read more (PubMed: 27385084) »
    • Xiong Y  et al. Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. Oncol Lett 11:99-104 (2016). Read more (PubMed: 26870175) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Following up my previous message, the lab confirmed that ab40809 was negative for IHC on formalin -fixed tissue. However, it was positive for ICC and flow cytometry on PFA-fixed samples. These application use relatively short fixation times compared to...

    Read More


    Sign up