Anti-ERK5 (phospho T219 + Y221) antibody (ab5686)
Key features and details
- Rabbit polyclonal to ERK5 (phospho T219 + Y221)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK5 (phospho T219 + Y221) antibody
See all ERK5 primary antibodies -
Description
Rabbit polyclonal to ERK5 (phospho T219 + Y221) -
Host species
Rabbit -
Specificity
Some cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to ERK5 (phospho T219 + Y221).
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Positive control
- WB: HEK293 cells transiently co-transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5 (MEK5D-D).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK5. The final product is generated by affinity chromatography using an ERK5-derived peptide that is phosphorylated at threonine 219 and tyrosine 221. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required.
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Notes |
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WB
1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required. |
Target
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Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. -
Tissue specificity
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus upon activation. - Information by UniProt
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Database links
- Entrez Gene: 5598 Human
- Entrez Gene: 23939 Mouse
- Omim: 602521 Human
- SwissProt: Q13164 Human
- SwissProt: Q9WVS8 Mouse
- Unigene: 150136 Human
- Unigene: 38172 Mouse
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Alternative names
- Big MAP kinase 1 antibody
- BMK 1 antibody
- BMK 1 kinase antibody
see all
Images
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Peptide Competition:
Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with the ab5686 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), the phosphopeptide derived from the corresponding region of ERK1&2 (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStarTM detection method. The data show that while there is some cross-reactivity with ERK1&
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab5686 has been referenced in 6 publications.
- Zhang Q et al. Isoflurane post-conditioning contributes to anti-apoptotic effect after cerebral ischaemia in rats through the ERK5/MEF2D signaling pathway. J Cell Mol Med 25:3803-3815 (2021). PubMed: 33621420
- Matsuura F et al. Extracellular signal-regulated kinases 2 (Erk2) and Erk5 in the central nervous system differentially contribute to central sensitization in male mice. J Neurosci Res 99:1666-1688 (2021). PubMed: 33729593
- Wang C et al. Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma. Exp Ther Med 21:470 (2021). PubMed: 33767765
- Hu ZG et al. MicroRNA-329-mediated PTTG1 downregulation inactivates the MAPK signaling pathway to suppress cell proliferation and tumor growth in cholangiocarcinoma. J Cell Biochem 120:9964-9978 (2019). PubMed: 30582202
- Song B et al. LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer. BMC Cancer 19:297 (2019). PubMed: 30940124
- Govarts C et al. Analysis of antibody reactivity in paired cerebrospinal fluid and serum of a relapsing remitting multiple sclerosis patient. Autoimmunity 42:699-704 (2009). IHC-Fr ; Human . PubMed: 19835487