Overview

  • Product name
  • Description
    Rabbit polyclonal to ERLIN1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ERLIN1 aa 245-275 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O75477

  • Positive control
    • HepG2 and A549 cell line lysates; Human liver tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
ICC/IF 1/50.

Target

  • Function
    Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs).
  • Tissue specificity
    Expressed in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary and small intestine.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Band 7 23-211 Keo4 (Interim) similar to C.elegans protein antibody
    • C10orf69 antibody
    • chromosome 10 open reading frame 69 antibody
    • Endoplasmic reticulum lipid raft-associated protein 1 antibody
    • ER lipid raft associated 1 antibody
    • Erlin 1 antibody
    • Erlin-1 antibody
    • ERLIN1 antibody
    • ERLN1_HUMAN antibody
    • KE04 antibody
    • KEO4 antibody
    • OTTHUMP00000059425 antibody
    • Protein KE04 antibody
    • SPFH domain containing protein 1 antibody
    • SPFH domain family, member 1 antibody
    • SPFH domain-containing protein 1 antibody
    • SPFH1 antibody
    • SPG62 antibody
    • Stomatin-prohibitin-flotillin-HflC/K domain containing protein 1 antibody
    • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-ERLIN1 antibody (ab171372) at 1/100 dilution

    Lane 1 : HepG2 cell line lysate
    Lane 2 : A549 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 39 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ERLIN1 with ab171372 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (HeLa cell)
Specification
HeLa cell
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Sep 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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