Overview

  • Product nameAnti-ERM / Etv5 antibody
    See all ERM / Etv5 primary antibodies
  • Description
    Rabbit polyclonal to ERM / Etv5
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 244-404 of Human ERM/ Etv5 (AAH07333)

  • Positive control
    • A431, Jurkat, Raji cell lysate, PC14 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab102010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22933516
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.

Target

Anti-ERM / Etv5 antibody images

  • Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ab102010 at 1/100 dilution.
  • Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 58 kDa

References for Anti-ERM / Etv5 antibody (ab102010)

This product has been referenced in:
  • Fanslow DA  et al. Genome editing in mouse spermatogonial stem/progenitor cells using engineered nucleases. PLoS One 9:e112652 (2014). ICC/IF . Read more (PubMed: 25409432) »
  • Lettice LA  et al. Opposing functions of the ETS factor family define Shh spatial expression in limb buds and underlie polydactyly. Dev Cell 22:459-67 (2012). ChIP . Read more (PubMed: 22340503) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spermatogonia)
Specification Spermatogonia
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton/PBS
Blocking step Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"