Overview

  • Product name
  • Description
    Rabbit polyclonal to ERM / Etv5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of human ERM/ Etv5

  • Positive control
    • ERM / Etv5 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

Images



  • Predicted band size : 58 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling ERM / Etv5 with ab75170 at 1.0µg/ml.


  • Predicted band size : 58 kDa
    Western blot analysis of Human fetal brain tissue lysate labeling ERM / Etv5 with ab75170 at 1.0µg/ml.
  • Anti-ERM / Etv5 antibody (ab75170) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Gel concentration: 12%

References

ab75170 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75170.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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