Overview

  • Product nameAnti-ERMAP antibody
    See all ERMAP primary antibodies
  • Description
    Rabbit polyclonal to ERMAP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 324-373 (PANGHWLLRQ SRGNEYEALT SPQTSFRLKE PPRCVGIFLD YEAGVISFYN) of human ERMAP (NP_061008).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionPossible role as a cell-adhesion or receptor molecule of erythroid cells.
  • Tissue specificityExpressed in erythroid-enriched bone marrow (at protein level). Highly expressed in bone marrow and to a lower extent in leukocytes, thymus, lymph node and spleen.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stageExpressed in fetal liver blood cells (at protein level). Highly expressed in fetal liver.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationCell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERMAP antibody
    • ERMAP_HUMAN antibody
    • Erythroblast membrane associated protein antibody
    • Erythroid membrane associated protein antibody
    • Erythroid membrane-associated protein antibody
    • hERMAP antibody
    • MGC118810 antibody
    • MGC118811 antibody
    • MGC118812 antibody
    • MGC118813 antibody
    • PRO2801 antibody
    • Radin blood group (Rd) antibody
    • Radin blood group antigen antibody
    • RD antibody
    • SC antibody
    • Scianna blood group (Sc) antibody
    • Scianna blood group antigen antibody
    see all

Anti-ERMAP antibody images

  • Anti-ERMAP antibody (ab83356) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ERMAP antibody (ab83356)

ab83356 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"