Overview

  • Product nameAnti-ERO1L antibody
    See all ERO1L primary antibodies
  • Description
    Mouse monoclonal to ERO1L
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DISQCGRRDC AVKPCQSDEV PDGIKSASYK YSEEANNLIE ECEQAERLGA VDESLSEETQ KAVLQWTKHD DSSDNFCEAD DIQSPEAEY, corresponding to amino acids 90-179 of Human ERO1L

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
    • Tissue specificityWidely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
    • Sequence similaritiesBelongs to the EROs family.
    • Post-translational
      modifications
      N-glycosylated.
      The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
    • Cellular localizationEndoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endoplasmic oxidoreductin 1 like protein antibody
      • Endoplasmic oxidoreductin-1-like protein antibody
      • Endoplasmic reticulum oxidoreductin 1-like antibody
      • ERO1 alpha antibody
      • ERO1 L antibody
      • ERO1 Lalpha antibody
      • ERO1 like protein alpha antibody
      • ERO1-alpha antibody
      • ERO1-L antibody
      • ERO1-L-alpha antibody
      • ERO1-like (S. cerevisiae) antibody
      • ERO1-like alpha antibody
      • ERO1-like protein alpha antibody
      • ERO1-like, S. cerevisiae, homolog of, alpha antibody
      • ERO1A antibody
      • ERO1A_HUMAN antibody
      • ERO1L antibody
      • ERO1LA antibody
      • Oxidoreductin 1 Lalpha antibody
      • Oxidoreductin-1-L-alpha antibody
      • PRO865 antibody
      • UNQ434 antibody
      see all

    Anti-ERO1L antibody images



    • Predicted band size : 54 kDa
      ERO1L antibody (ab57177) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-ERO1L antibody (ab57177)

    This product has been referenced in:
    • Zhang L  et al. Different interaction modes for protein-disulfide isomerase (PDI) as an efficient regulator and a specific substrate of endoplasmic reticulum oxidoreductin-1a (Ero1a). J Biol Chem 289:31188-99 (2014). Read more (PubMed: 25258311) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab57177.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"