• Product name
  • Description
    Rabbit polyclonal to ERp29
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ERp29.

    (Peptide available as ab42000.)

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue Lysates: Testis and Liver.



Our Abpromise guarantee covers the use of ab40982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


  • Relevance
    Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI’s thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
  • Cellular localization
    Endoplasmic reticulum, Cell surface.
  • Database links
  • Alternative names
    • C12orf8 antibody
    • Chromosome 12 open reading frame 8 antibody
    • Endoplasmic reticulum lumenal protein ERp28 antibody
    • Endoplasmic reticulum protein 29 antibody
    • Endoplasmic reticulum protein 29 isoform 1 antibody
    • Endoplasmic reticulum protein ERp29 antibody
    • Endoplasmic reticulum resident protein 28 antibody
    • Endoplasmic reticulum resident protein 29 antibody
    • Endoplasmic reticulum resident protein 31 antibody
    • Epididymis secretory protein Li 107 antibody
    • ERp28 antibody
    • ERP29_HUMAN antibody
    • ERp31 antibody
    • HEL S 107 antibody
    • PDI-DB antibody
    • PDIA9 antibody
    • Protein disulfide isomerase family A member 9 antibody
    see all

Anti-ERp29 antibody images

  • All lanes : Anti-ERp29 antibody (ab40982) at 2 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • IHC image of ERp29 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40982, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-ERp29 antibody (ab40982)

ab40982 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% Triton X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 13 2008


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