Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyan
ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-ERp57 antibody (ab13507)
All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution
Lane 1 : Molecular weight marker Lane 2 : Cell lysates prepared from CHO-K1 cells Lane 3 : Cell lysates prepared from human liver microsomes Lane 4 : Cell lysates prepared from rat liver microsomes Lane 5 : Cell lysates prepared from mouse liver microsomes
Predicted band size : 57 kDa
References for Anti-ERp57 antibody (ab13507)
This product has been referenced in:
Petrosyan A et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem290:6256-69 (2015).
Read more (PubMed: 25605727) »
Li J et al. Identification and characterization of an oocyte factor required for sperm decondensation in pig. Reproduction148:367-75 (2014).
Read more (PubMed: 25030891) »