Overview

  • Product name
  • Description
    Rabbit polyclonal to ERp57
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 202-453 of Human ERp57 (UniProt ID: P30101).

  • Positive control
    • HeLa, HepG2 whole cell lysate and Mouse brain lysate; BT474 xenograft tissue; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity
    Detected in the flagellum and head region of spermatozoa (at protein level).
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 58 kDa glucose regulated protein antibody
    • 58 kDa glucose-regulated protein antibody
    • 58 kDa microsomal protein antibody
    • Disulfide isomerase ER 60 antibody
    • Disulfide isomerase ER-60 antibody
    • Endoplasmic reticulum resident protein 57 antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • ER p57 antibody
    • ER protein 57 antibody
    • ER protein 60 antibody
    • ERp 57 antibody
    • ERp57 antibody
    • ERp60 antibody
    • ERp61 antibody
    • Glucose Regulated Protein 58 Kd antibody
    • GRP 57 antibody
    • GRP 58 antibody
    • GRP57 antibody
    • HsT17083 antibody
    • p58 antibody
    • PDIA 3 antibody
    • PDIA3 antibody
    • PDIA3_HUMAN antibody
    • Phospholipase C alpha antibody
    • PI PLC antibody
    • Protein disulfide isomerase A3 antibody
    • Protein disulfide isomerase family A member 3 antibody
    • Protein disulfide-isomerase A3 antibody
    see all

Images

  • Anti-ERp57 antibody (ab153975) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Anti-ERp57 antibody (ab153975) at 1/1000 dilution + Mouse brain whole cell lysate
    at 50 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells labeling ERp57 with ab153975 at 1/200 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded BT474 xenograft tissue labeling ERp57 with ab153975 at 1/100 dilution.

References

ab153975 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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