Anti-ERp57 antibody (ab88098)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, Chinese hamster
Predicted to work with: Sheep, Goat, Cow, Baboon, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Tissue specificity
Detected in the flagellum and head region of spermatozoa (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 100689343 Chinese hamster
- Entrez Gene: 281803 Cow
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Entrez Gene: 100174305 Orangutan
- Omim: 602046 Human
- SwissProt: P38657 Cow
- SwissProt: P30101 Human
see all -
Alternative names
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
Images
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All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PDIA3 (ERp57) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaLanes 1 - 2: Merged signal (red and green). Green - ab88098 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab88098 was shown to recognize PDIA3 in wild-type HAP1 cells as signal was lost at the expected MW in PDIA3 (ERp57) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDIA3 (ERp57) knockout samples were subjected to SDS-PAGE. Ab88098 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 5 : CHO-K1 cell lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab88098 has not yet been referenced specifically in any publications.