Endoplasmic reticulum resident protein 57 antibody
Endoplasmic reticulum resident protein 60 antibody
ER p57 antibody
ER protein 57 antibody
ER protein 60 antibody
ERp 57 antibody
Glucose Regulated Protein 58 Kd antibody
GRP 57 antibody
GRP 58 antibody
PDIA 3 antibody
Phospholipase C alpha antibody
PI PLC antibody
Protein disulfide isomerase A3 antibody
Protein disulfide isomerase family A member 3 antibody
Protein disulfide-isomerase A3 antibody
Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg) Lane 3: HepG2 whole cell lysate (20 µg) Lane 4: Hek293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154191 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab154191 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154191 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One11:e0146530 (2016).
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