Validated using a knockout cell line
Recombinant
RabMAb

Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Overview

  • Product name
    Anti-ERp57 antibody [EPR10679(B)]
    See all ERp57 primary antibodies
  • Description
    Rabbit monoclonal [EPR10679(B)] to ERp57
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ERp57 (UniProt: P30101).

  • Positive control
    • HeLa, HepG2, Daudi and 293T cell lysates; Human pancreas and stomach tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10679(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Tissue specificity
      Detected in the flagellum and head region of spermatozoa (at protein level).
    • Sequence similarities
      Belongs to the protein disulfide isomerase family.
      Contains 2 thioredoxin domains.
    • Cellular localization
      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 58 kDa glucose regulated protein antibody
      • 58 kDa glucose-regulated protein antibody
      • 58 kDa microsomal protein antibody
      • Disulfide isomerase ER 60 antibody
      • Disulfide isomerase ER-60 antibody
      • Endoplasmic reticulum resident protein 57 antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • ER p57 antibody
      • ER protein 57 antibody
      • ER protein 60 antibody
      • ERp 57 antibody
      • ERp57 antibody
      • ERp60 antibody
      • ERp61 antibody
      • Glucose Regulated Protein 58 Kd antibody
      • GRP 57 antibody
      • GRP 58 antibody
      • GRP57 antibody
      • HsT17083 antibody
      • p58 antibody
      • PDIA 3 antibody
      • PDIA3 antibody
      • PDIA3_HUMAN antibody
      • Phospholipase C alpha antibody
      • PI PLC antibody
      • Protein disulfide isomerase A3 antibody
      • Protein disulfide isomerase family A member 3 antibody
      • Protein disulfide-isomerase A3 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab154197 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab154197 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154197 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ERp57 with ab154197 at 1/50 dilution
    • All lanes : Anti-ERp57 antibody [EPR10679(B)] (ab154197) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ERp57 with ab154197 at 1/50 dilution
    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154197 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154197 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154197 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154197 showing +ve staining.

    References

    ab154197 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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