Endoplasmic reticulum resident protein 57 antibody
Endoplasmic reticulum resident protein 60 antibody
ER p57 antibody
ER protein 57 antibody
ER protein 60 antibody
ERp 57 antibody
Glucose Regulated Protein 58 Kd antibody
GRP 57 antibody
GRP 58 antibody
PDIA 3 antibody
Phospholipase C alpha antibody
PI PLC antibody
Protein disulfide isomerase A3 antibody
Protein disulfide isomerase family A member 3 antibody
Protein disulfide-isomerase A3 antibody
Western blot - Anti-ERp57 antibody [MaP.Erp57] (ab13506)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg) Lane 3: HepG2 whole cell lysate (20 µg) Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab13506 observed at 57 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab13506 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab13506 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 0.5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ICC/IF image of ab13506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab13506 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - ERp57 antibody [MaP.Erp57] (ab13506)This image is courtesy of an Abreview submitted by Pawel Mazur
ERp57 antibody (ab13506) at 1/1000 dilution (in PBST) for 8 hours at 4°C + lysate of mouse of pancreatic cancer cell line at 25 µg.
An HRP conjugated Sheep anti-mouse IgG polyclonal at 1/5000 dilution developed using the ECL technique
Performed under non-reducing conditions.
Exposure time: 1 minute
Blocking Step: 10% Milk for 1 hour at room temperature.
Immunohistochemistry (Frozen sections) - ERp57 antibody [MaP.Erp57] (ab13506)This image is courtesy of an anonymous Abreview
ab13506 staining ERp57 in Mouse duodenum, pancreas, and pancreatic neoplastic tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone:methanol (1:1), permeabilized with PBS + Triton X-100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI.
Overlay histogram showing HeLa cells stained with ab13506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13506, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Hsu SL et al. Extracorporeal Shockwave Therapy Enhances Expression of Pdia-3 Which Is a Key Factor of the 1a,25-Dihydroxyvitamin D 3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis of the Knee. Int J Med Sci14:1220-1230 (2017).
Read more (PubMed: 29104478) »
Takata H et al. Increased expression of PDIA3 and its association with cancer cell proliferation and poor prognosis in hepatocellular carcinoma. Oncol Lett12:4896-4904 (2016).
Read more (PubMed: 28101228) »