Customer reviews and FAQs

Overview

  • Product name
    Anti-ERp72 antibody
    See all ERp72 primary antibodies
  • Description
    Rabbit polyclonal to ERp72
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Monkey
  • Immunogen

    Synthetic peptide: FIDEHATKRSRTKEEL, corresponding to amino acids 623-638 of Mouse ERp72, conjugated to KLH and with an added cysteine residue

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Liver microsome extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab82587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Calcium binding protein intestinal related antibody
    • Endoplasmic reticulum resident protein 70 antibody
    • Endoplasmic reticulum resident protein 72 antibody
    • EPR70 antibody
    • EPR72 antibody
    • ER protein 70 antibody
    • ER protein 72 antibody
    • ERP 70 antibody
    • ERp 72 antibody
    • ERp-72 antibody
    • ERp70 antibody
    • ERp72 antibody
    • PDIA 4 antibody
    • Pdia4 antibody
    • PDIA4_HUMAN antibody
    • PDIR antibody
    • Protein disulfide isomerase A4 antibody
    • Protein disulfide isomerase associated 4 antibody
    • Protein disulfide isomerase family A member 4 antibody
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A4 antibody
    • Protein ERp 72 antibody
    • Protein ERp72 antibody
    see all

Anti-ERp72 antibody images

  • Western blot - ERp72 antibody (ab82587)
    Western blot - ERp72 antibody (ab82587)
    All lanes : Anti-ERp72 antibody (ab82587) at 1/1000 dilution

    Lane 1 : Rat liver microsome extract
    Lane 2 : Mouse liver microsome extract
    Lane 3 : Human liver microsome extract


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-ERp72 antibody (ab82587)

ab82587 has not yet been referenced specifically in any publications.

Publishing research using ab82587? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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2 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-ERp72 antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Duodenum tissue)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Duodenum tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted May 15 2017

Western blot abreview for Anti-ERp72 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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