Overview

  • Product name
    Anti-ERp72 antibody
    See all ERp72 primary antibodies
  • Description
    Rabbit polyclonal to ERp72
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Monkey
  • Immunogen

    Synthetic peptide: FIDEHATKRSRTKEEL, corresponding to amino acids 623-638 of Mouse ERp72, conjugated to KLH and with an added cysteine residue

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Liver microsome extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab82587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Calcium binding protein intestinal related antibody
    • Endoplasmic reticulum resident protein 70 antibody
    • Endoplasmic reticulum resident protein 72 antibody
    • EPR70 antibody
    • EPR72 antibody
    • ER protein 70 antibody
    • ER protein 72 antibody
    • ERP 70 antibody
    • ERp 72 antibody
    • ERp-72 antibody
    • ERp70 antibody
    • ERp72 antibody
    • PDIA 4 antibody
    • Pdia4 antibody
    • PDIA4_HUMAN antibody
    • PDIR antibody
    • Protein disulfide isomerase A4 antibody
    • Protein disulfide isomerase associated 4 antibody
    • Protein disulfide isomerase family A member 4 antibody
    • protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A4 antibody
    • Protein ERp 72 antibody
    • Protein ERp72 antibody
    see all

Images

  • Western blot - ERp72 antibody (ab82587)
    Western blot - ERp72 antibody (ab82587)
    All lanes : Anti-ERp72 antibody (ab82587) at 1/1000 dilution

    Lane 1 : Rat liver microsome extract
    Lane 2 : Mouse liver microsome extract
    Lane 3 : Human liver microsome extract

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab82587? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-ERp72 antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Duodenum tissue)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Duodenum tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted May 15 2017

Western blot abreview for Anti-ERp72 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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