Anti-MIG-6 antibody (ab227944)
Key features and details
- Rabbit polyclonal to MIG-6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MIG-6 antibody
See all MIG-6 primary antibodies -
Description
Rabbit polyclonal to MIG-6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human MIG-6 (internal sequence). The exact sequence is proprietary.
Database link: Q9UJM3 -
Positive control
- WB: HCT 116 whole cell lysate. IHC-P: Mouse placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development. -
Sequence similarities
Belongs to the MIG6 family. -
Domain
The EGFR-binding region prevents binding of a cyclin-like activator to the EGFR kinase domain, and thereby keeps EGFR in an inactive conformation. Also maintains EGFR in an inactive conformation by preventing formation of an asymmetric homodimer. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes. - Information by UniProt
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Database links
- Entrez Gene: 54206 Human
- Entrez Gene: 74155 Mouse
- Entrez Gene: 722072 Rhesus monkey
- Omim: 608069 Human
- SwissProt: Q9UJM3 Human
- SwissProt: Q99JZ7 Mouse
- Unigene: 605445 Human
- Unigene: 318841 Mouse
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Alternative names
- ERBB receptor feedback inhibitor 1 antibody
- ERRFI_HUMAN antibody
- Errfi1 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab227944 has been referenced in 5 publications.
- Wei Y et al. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression. J Bone Miner Res 37:1012-1023 (2022). PubMed: 35191092
- Wang M et al. Glioma exosomal microRNA-148a-3p promotes tumor angiogenesis through activating the EGFR/MAPK signaling pathway via inhibiting ERRFI1. Cancer Cell Int 20:518 (2020). PubMed: 33117083
- Ma P et al. A novel role of the miR-152-3p/ERRFI1/STAT3 pathway modulates the apoptosis and inflammatory response after acute kidney injury. J Biochem Mol Toxicol N/A:e22540 (2020). PubMed: 32583487
- Pan R et al. Inhibition of KHSRP sensitizes colorectal cancer to 5-fluoruracil through miR-501-5p-mediated ERRFI1 mRNA degradation. J Cell Physiol N/A:N/A (2019). PubMed: 31313286
- Liang R et al. High expression of estrogen-related receptor a is significantly associated with poor prognosis in patients with colorectal cancer. Oncol Lett 15:5933-5939 (2018). PubMed: 29552224