Erythropoietin (EPO) Human ELISA Kit (ab119522)

Overview

  • Product nameErythropoietin (EPO) Human ELISA Kit
    See all Erythropoietin (EPO) kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3.4%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.4 mIU/ml
  • Range
    1.6 mIU/ml - 100 mIU/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 81 71% - 93%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Erythropoietin (EPO) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Erythropoietin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    Erythropoietin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Erythropoietin detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Erythropoietin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionErythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
  • Tissue specificityProduced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Involvement in diseaseGenetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2) [MIM:612623]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similaritiesBelongs to the EPO/TPO family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • EP
    • EPO
    • EPO_HUMAN
    • Epoetin
    • Erythropoietin
    • MVCD2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Erythropoietin (EPO) Human ELISA Kit images

  • Human Erythropoietin standard curve: mean of duplicates (+/- SD)
  • Human Erythropoietin measured in biological fluids showing quantity (mIU) per mL of tested sample.
  • Representative Standard Curve using ab119522.

Protocols

References for Erythropoietin (EPO) Human ELISA Kit (ab119522)

ab119522 has not yet been referenced specifically in any publications.

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Thank you for your enquiry regarding ab119522.

This product has been designed to detect human Erythropoietin (EPO) and it has only been tested on human samples; we do not have any information regarding its cross-reactivity with other species....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"