Overview

  • Product name
    Anti-Esa1 antibody
  • Description
    Rabbit polyclonal to Esa1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-235 of Human Esa1 (UniProt ID: Q14254).

  • Positive control
    • A549, HeLa, HepG2, HCT116, mouse brain whole cell lysate; BT474 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • Tissue specificity
    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • Sequence similarities
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization
    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECS 1 antibody
    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin-2 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody) antibody
    see all

Images

  • Anti-Esa1 antibody (ab154110) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Anti-Esa1 antibody (ab154110) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded BT474 xenograft tissue labeling Esa1 with ab154110 at 1/100 dilution.

References

ab154110 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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