Anti-ESAM antibody (ab73988)

Overview

  • Product name
  • Description
    Rabbit polyclonal to ESAM
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminal domain of human ESAM

Properties

Applications

Our Abpromise guarantee covers the use of ab73988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 41 kDa.
IHC-Fr 1/50 - 1/100.

Target

References

ab73988 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Sorry I was not able to take your email last week. I was not in the office. I am very sorry that the two anti-ESAM antibodies did not show the staining we had hoped for, it may be that as you say that the epitope presented by the protein (even thou...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon Tumour)
Specification
Colon Tumour
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 15 2011

Thank you for contacting us. We currently have two antibodies directed towards human EMSA, ab74777 and ab73988. Both are rabbit polyclonals. ab74777 like the antibody you have previously used is directed against the extracellular domain of ESAM...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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