Anti-ESAT6 antibody (ab45073)
Key features and details
- Rabbit polyclonal to ESAT6
- Suitable for: WB
- Reacts with: Mycobacterium bovis
- Isotype: IgG
Overview
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Product name
Anti-ESAT6 antibody -
Description
Rabbit polyclonal to ESAT6 -
Host species
Rabbit -
Specificity
The rabbit polyclonal antibody to EsaT-6 recognizes the EsaT-6 (Rv3875) protein of Mycobacterium tuberculosis and Mycobacterium bovis.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mycobacterium bovis, Mycobacterium tuberculosis -
Immunogen
Recombinant full length protein corresponding to ESAT6.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa.
Use under reducing conditions. |
Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa. Use under reducing conditions. |
Target
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Relevance
The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response. -
Cellular localization
Secreted -
Alternative names
- 6 kDa early secretory antigenic target antibody
- 6 kDa early secretory antigenic target ESXA antibody
- ESAT 6 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab45073 has been referenced in 4 publications.
- Yang H et al. Interception of host fatty acid metabolism by mycobacteria under hypoxia to suppress anti-TB immunity. Cell Discov 7:90 (2021). PubMed: 34608123
- Sepulveda D et al. Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem. Sensors (Basel) 17:N/A (2017). PubMed: 28937645
- Tan K et al. Comparison of BCG prime-DNA booster and rBCG regimens for protection against tuberculosis. Hum Vaccin Immunother 10:391-8 (2014). PubMed: 24192709
- Nguyen YH et al. Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay. Sci Rep 2:635 (2012). Functional Studies . PubMed: 22957139