Overview

  • Product nameAnti-ESAT6 antibody
    See all ESAT6 primary antibodies
  • Description
    Rabbit polyclonal to ESAT6
  • SpecificityThis antibody reacts with recombinant ESAT6 produced in E.coli.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Mycobacterium tuberculosis (strain H37Rv) and Mycobacterium bovis. Not yet tested in other species.
  • Immunogen

    Full length protein (Rv3875) from Mycobacterium tubercolosis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). Use under reducing conditions.

Target

  • RelevanceThe ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • Cellular localizationSecreted
  • Alternative names
    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

References for Anti-ESAT6 antibody (ab45073)

This product has been referenced in:
  • Nguyen YH  et al. Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay. Sci Rep 2:635 (2012). Functional Studies . Read more (PubMed: 22957139) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 0.5 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Specification Cell lysate, supernatant
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
Total protein in input 1000 µg
Specification M. tuberculosis culture filtrate
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Jul 11 2012

Thank you for contacting us.
Your credit note ID is ***.
I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn...

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Thank you very much for your reply.

I can certainly arrange a credit or refund for you for this purchase. A credit can be held on your account and used against a future order, or we can issue a full refund. Do you have any information about y...

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Thank you for submitting your recent review of ab45073. I am sorry to see that the results with this antibody were so poor.

We do fully guarantee this antibody to work in Western blot with M tuberculosis samples, so if you would like, I can s...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount 15 µg
Specification M.tuberculosis culture filtrate
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 02 2012

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"