Overview

  • Product name
  • Description
    Rabbit polyclonal to ESCO2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within internal sequence amino acids 135-165 of Human ESCO2 (NP_001017420.1).

  • Positive control
    • Mouse stomach tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab107277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 68 kDa.

Target

  • Relevance
    ESCO2 is an acetyltransferase required for the establishment of sister chromatid cohesion, and couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Defects in ESCO2 are the cause of Roberts syndrome (RBS), a rare autosomal recessive disorder characterized by pre- and postnatal growth retardation, microcephaly, bilateral cleft lip and palate, and mesomelic symmetric limb reduction. Severely affected infants may be stillborn or die shortly after birth. RBS chromosomes have a lack of cohesion involving the heterochromatic C-banding regions around centromeres and the distal portion of the long arm of the Y chromosome (known as premature centromere separation, heterochromatin repulsion or puffing, or RS effect). Defects in ESCO2 are also the cause of SC phocomelia syndrome, also known as SC pseudothalidomide syndrome. SC phocomelia syndrome has a milder phenotype than RBS, with a lesser degree of symmetric limb reduction and additionally includes flexion contractures of various joints, midfacial hemangioma, hypoplastic cartilage of ears and nose, scant silvery-blond hair, and cloudy corneae. Although microcephaly is present, mental retardation may be mild and survival into adulthood is common.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CTF7, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • ECO1 homolog 2 antibody
    • ECO1, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • EFO2 antibody
    • ESO1, S. POMBE, HOMOLOG OF, 2 antibody
    • ESTABLISHMENT FACTOR ORTHOLOG 2; EFO2 antibody
    • Establishment of cohesion 1 homolog 2 antibody
    • Establishment of cohesion 1 homolog 2 (S. cerevisiae) antibody
    • N acetyltransferase ESCO2 antibody
    • RBS antibody
    • Roberts syndrome antibody
    see all

Images

  • Anti-ESCO2 antibody (ab107277) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size : 68 kDa

References

ab107277 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 11 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
100 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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