Overview

  • Product nameAnti-ESCO2 antibody
    See all ESCO2 primary antibodies
  • Description
    Rabbit polyclonal to ESCO2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    A synthetic peptide corresponding to a region between residue 200 and 250 of human ESCO2 (NP_001017420.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceESCO2 is an acetyltransferase required for the establishment of sister chromatid cohesion, and couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Defects in ESCO2 are the cause of Roberts syndrome (RBS), a rare autosomal recessive disorder characterized by pre- and postnatal growth retardation, microcephaly, bilateral cleft lip and palate, and mesomelic symmetric limb reduction. Severely affected infants may be stillborn or die shortly after birth. RBS chromosomes have a lack of cohesion involving the heterochromatic C-banding regions around centromeres and the distal portion of the long arm of the Y chromosome (known as premature centromere separation, heterochromatin repulsion or puffing, or RS effect). Defects in ESCO2 are also the cause of SC phocomelia syndrome, also known as SC pseudothalidomide syndrome. SC phocomelia syndrome has a milder phenotype than RBS, with a lesser degree of symmetric limb reduction and additionally includes flexion contractures of various joints, midfacial hemangioma, hypoplastic cartilage of ears and nose, scant silvery-blond hair, and cloudy corneae. Although microcephaly is present, mental retardation may be mild and survival into adulthood is common.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • CTF7, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • ECO1 homolog 2 antibody
    • ECO1, S. CEREVISIAE, HOMOLOG OF, 2 antibody
    • EFO2 antibody
    • ESO1, S. POMBE, HOMOLOG OF, 2 antibody
    • ESTABLISHMENT FACTOR ORTHOLOG 2; EFO2 antibody
    • Establishment of cohesion 1 homolog 2 antibody
    • Establishment of cohesion 1 homolog 2 (S. cerevisiae) antibody
    • N acetyltransferase ESCO2 antibody
    • RBS antibody
    • Roberts syndrome antibody
    see all

Anti-ESCO2 antibody images

  • All lanes : Anti-ESCO2 antibody (ab86003) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of human ESCO2 by immunoprecipitation. 1mg HeLa Detection of ESCO2 by Western Blot of Immunprecipitate. ab86003 at 1µg/ml staining ESCO2 in HeLa whole cell lysate immunoprecipitated using ab86003 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-ESCO2 antibody (ab86003)

ab86003 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Formaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 40 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"