Overview

  • Product nameAnti-ESM1 antibody
    See all ESM1 primary antibodies
  • Description
    Rabbit polyclonal to ESM1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-184 of Human ESM1 (NP_008967.1).

  • Positive control
    • Human kidney tissue lysate; ESM1 transfected 293T cell line lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab103590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMay have potent implications in lung endothelial cell-leukocyte interactions.
  • Tissue specificityExpressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
  • Post-translational
    modifications
    May contain intrachain disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endocan antibody
    • Endothelial cell specific molecule 1 antibody
    • Endothelial cell-specific molecule 1 antibody
    • ESM 1 antibody
    • ESM 1 secretory protein antibody
    • ESM-1 antibody
    • Esm1 antibody
    • ESM1 secretory protein antibody
    • ESM1_HUMAN antibody
    see all

Anti-ESM1 antibody images

  • Anti-ESM1 antibody (ab103590) at 1/500 dilution + Human kidney tissue lysate at 50 µg

    Predicted band size : 20 kDa
  • All lanes : Anti-ESM1 antibody (ab103590) at 1/500 dilution

    Lane 1 : ESM1 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 20 kDa
  • ab103590 staining ESM1 in the Human HUVEC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit monoclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-ESM1 antibody (ab103590)

ab103590 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEC)
Specification HUVEC
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"