Overview

  • Product name
  • Description
    Rabbit polyclonal to ESRP2
  • Specificity
    ab113486 is predicted to not cross-react with other ESRP family members. At least three isoforms of ESRP2 are known to exist; ab113486 will detect all three.
  • Tested applications
    Suitable for: ICC/IF, WB, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide containing a 17 residue sequence from the C terminal region of Human ESRP2 (GenBank accession no. NP_079215).

  • Positive control
    • Human lung tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
  • Tissue specificity
    Epithelial cell-specific.
  • Sequence similarities
    Belongs to the ESRP family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epithelial splicing regulatory protein 2 antibody
    • ESRP 2 antibody
    • ESRP2 antibody
    • ESRP2_HUMAN antibody
    • FLJ21918 antibody
    • FLJ22248 antibody
    • RBM35B antibody
    • RNA binding motif protein 35A antibody
    • RNA binding motif protein 35B antibody
    • RNA binding protein 35B antibody
    • RNA-binding motif protein 35B antibody
    • RNA-binding protein 35B antibody
    see all

Images

  • Immunofluorescence of ESRP2 in Human lung tissue cells with ab113486 at 20 ug/mL.
  • Anti-ESRP2 antibody (ab113486) at 1 µg/ml + Human lung tissue lysate at 15 µg

    Predicted band size : 78 kDa
  • Immunohistochemical analysis of Human lung tissue, staining ESRP2 with ab113486 at 5 µg/ml.

References

This product has been referenced in:
  • Deloria AJ  et al. Epithelial splicing regulatory protein 1 and 2 paralogues correlate with splice signatures and favorable outcome in human colorectal cancer. Oncotarget 7:73800-73816 (2016). Human . Read more (PubMed: 27650542) »
  • Ishii H  et al. Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms. J Biol Chem 289:27386-99 (2014). Read more (PubMed: 25143390) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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