The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
FunctionmRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Tissue specificityEpithelial cell-specific.
Sequence similaritiesBelongs to the ESRP family. Contains 3 RRM (RNA recognition motif) domains.
Immunohistochemical analysis of Human lung tissue, staining ESRP2 with ab113486 at 5 µg/ml.
References for Anti-ESRP2 antibody (ab113486)
This product has been referenced in:
Deloria AJ et al. Epithelial splicing regulatory protein 1 and 2 paralogues correlate with splice signatures and favorable outcome in human colorectal cancer. Oncotarget7:73800-73816 (2016).
Read more (PubMed: 27650542) »
Ishii H et al. Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms. J Biol Chem289:27386-99 (2014).
Read more (PubMed: 25143390) »