Overview

  • Product nameAnti-ESRP2 antibody
    See all ESRP2 primary antibodies
  • Description
    Rabbit polyclonal to ESRP2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (ATAGALGRDL GSDETDLILL VWQVVEPRSR QVGTLHKSLV RAEAAALSTQ) of Human ESRP2 (NP_079215).

  • Positive control
    • Hela cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionmRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
  • Tissue specificityEpithelial cell-specific.
  • Sequence similaritiesBelongs to the ESRP family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epithelial splicing regulatory protein 2 antibody
    • ESRP 2 antibody
    • ESRP2 antibody
    • ESRP2_HUMAN antibody
    • FLJ21918 antibody
    • FLJ22248 antibody
    • RBM35B antibody
    • RNA binding motif protein 35A antibody
    • RNA binding motif protein 35B antibody
    • RNA binding protein 35B antibody
    • RNA-binding motif protein 35B antibody
    • RNA-binding protein 35B antibody
    see all

Anti-ESRP2 antibody images



  • Predicted band size : 78 kDa
    Western blot analysis of HepG2 cell lysate labeling ESRP2 with ab83034 at 1.0µg/ml.


  • Predicted band size : 78 kDa
    Western blot analysis of HeLa cell lysate labeling ESRP2 with ab83034 at 1.0µg/ml.
  • Anti-ESRP2 antibody (ab83034) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Gel concentration: 12%

References for Anti-ESRP2 antibody (ab83034)

ab83034 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83034.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"