Overview

  • Product nameAnti-EST1A antibody
  • Description
    Rabbit polyclonal to EST1A
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human EST1A.

    (Peptide available as ab98260.)

  • Positive control
    • This antibody gave a positive signal in both Jurkat and HepG2 nuclear lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24554440

Target

  • RelevanceTelomerase-binding protein EST1A is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It has been shown to promote the ability of TERT to elongate telomeres and may have some endonuclease function.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • C17orf31 antibody
    • EST1-like protein A antibody
    • Ever shorter telomeres 1A antibody
    • hSmg5/7a antibody
    • KIAA0732 antibody
    • Smg-6 homolog antibody
    • SMG6 antibody
    • Telomerase subunit EST1A antibody
    • Telomerase-binding protein EST1A antibody
    see all

Anti-EST1A antibody images

  • All lanes : Anti-EST1A antibody (ab87539) at 1 µg/ml

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 160 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,46 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab87539 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab87539, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100% methanol fixed (5 min) MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data

References for Anti-EST1A antibody (ab87539)

This product has been referenced in:
  • Durand S  et al. Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. Nat Commun 7:12434 (2016). WB . Read more (PubMed: 27511142) »
  • Li T  et al. Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay. EMBO J N/A:N/A (2015). WB ; Mouse . Read more (PubMed: 25770585) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tangliang Li

Verified customer

Submitted May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"