Overview

  • Product name
    Anti-EST1A antibody
  • Description
    Rabbit polyclonal to EST1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human EST1A.

    (Peptide available as ab98260.)

  • Positive control
    • This antibody gave a positive signal in both Jurkat and HepG2 nuclear lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24554440

Target

  • Relevance
    Telomerase-binding protein EST1A is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It has been shown to promote the ability of TERT to elongate telomeres and may have some endonuclease function.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • C17orf31 antibody
    • EST1-like protein A antibody
    • Ever shorter telomeres 1A antibody
    • hSmg5/7a antibody
    • KIAA0732 antibody
    • Smg-6 homolog antibody
    • SMG6 antibody
    • Telomerase subunit EST1A antibody
    • Telomerase-binding protein EST1A antibody
    see all

Images

  • All lanes : Anti-EST1A antibody (ab87539) at 1 µg/ml

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 160 kDa
    Observed band size: 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 120 kDa, 46 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    The predicted Mw for Human Telomerase-binding protein EST1A is 160 kDa. We are unsure why we are seeing the banding pattern at kDa however Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab87539 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab87539, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100% methanol fixed (5 min) MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data

References

This product has been referenced in:
  • Huang L  et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). Read more (PubMed: 29334995) »
  • Muller M & Glaunsinger BA Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases. PLoS Pathog 13:e1006593 (2017). WB . Read more (PubMed: 28841715) »

See all 10 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tangliang Li

Verified customer

Submitted May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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