|Control Sera||32||< 9.7%|
|Control Sera||36||< 10%|
Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Estriol IgG Coated Microplate (12 x 8 wells)||1 unit|
|Control A||1 x 1ml|
|Control B||1 x 1ml|
|Cover foils||1 unit|
|Estriol Standard 0 - 0 ng/mL||1 x 1ml|
|Estriol Standard 1 – 2 ng/mL||1 x 1ml|
|Estriol Standard 2 – 20 ng/mL||1 x 1ml|
|Estriol Standard 3 – 80 ng/mL||1 x 1ml|
|Estriol Standard 4 – 200 ng/mL||1 x 1ml|
|Estriol-HRP Conjugate||1 x 22ml|
|Incubation buffer||1 x 30ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
|Wash Solution||1 x 50ml|
Our Abpromise guarantee covers the use of ab108675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab108675 has not yet been referenced specifically in any publications.