Overview

  • Product nameEstriol ELISA Kit
    See all Estriol kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Control Sera 32 < 9.7%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 36 < 10%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    0.22 ng/ml
  • Range
    2 ng/ml - 200 ng/ml
  • Recovery

    94.88 %

  • Assay durationMultiple steps standard assay
  • Product overview

    Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Estriol. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • Oestriol

Applications

Our Abpromise guarantee covers the use of ab108675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Protocols

References for Estriol ELISA Kit (ab108675)

ab108675 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"