Anti-Estrogen-induced gene 121 protein antibody (ab156275)

Overview

  • Product name
    Anti-Estrogen-induced gene 121 protein antibody
    See all Estrogen-induced gene 121 protein primary antibodies
  • Description
    Rabbit polyclonal to Estrogen-induced gene 121 protein
  • Host species
    Rabbit
  • Specificity
    ab156275 is predicted to not cross-react with other UPF0577 protein family members.
  • Tested applications
    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to 18 amino acids at the C terminus of Human Estrogen-induced gene 121 protein (NP_065826).

  • Positive control
    • MCF7 cell lysate; MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156275 is affinity chromatography purified via peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa.
ICC Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    May play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma.
  • Tissue specificity
    Expressed in normal endometrium but overexpressed in endometroid tumors.
  • Sequence similarities
    Belongs to the UPF0577 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EIG121 antibody
    • Estrogen induced gene 121 antibody
    • Estrogen-induced gene 121 protein antibody
    • Hypothetical protein LOC57535 antibody
    • K1324_HUMAN antibody
    • KIAA1324 antibody
    • MABA1 antibody
    • MGC150624 antibody
    • UPF0577 protein KIAA1324 antibody
    see all

Images

  • Immunofluorescence of Estrogen-induced gene 121 protein in MCF7 cells with ab156275 at 20 ug/mL.
  • All lanes : Anti-Estrogen-induced gene 121 protein antibody (ab156275) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 111 kDa

  • Immunocytochemical analysis of MCF7 cells labeling Estrogen-induced gene 121 protein with ab156275 at 5 µg/ml.

References

This product has been referenced in:
  • Liu H  et al. PI3K/AKT/mTOR pathway promotes progestin resistance in endometrial cancer cells by inhibition of autophagy. Onco Targets Ther 10:2865-2871 (2017). WB ; Human . Read more (PubMed: 28652768) »
  • Engel BJ  et al. Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures. Adv Healthc Mater 4:1664-74 (2015). Read more (PubMed: 26059746) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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