Anti-Estrogen-induced gene 121 protein antibody (ab81247)

Overview

  • Product nameAnti-Estrogen-induced gene 121 protein antibody
    See all Estrogen-induced gene 121 protein primary antibodies
  • Description
    Rabbit polyclonal to Estrogen-induced gene 121 protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (CKPCAEGRYS LGTGIRFDEW DELPHGFASL SANMELDDSA AESTGNCTSS) of Human Estrogen-induced gene 121 protein (NP_065826)

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 111 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

Anti-Estrogen-induced gene 121 protein antibody images

  • Anti-Estrogen-induced gene 121 protein antibody (ab81247) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 111 kDa
    Observed band size : 111 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Estrogen-induced gene 121 protein antibody (ab81247)

ab81247 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"