Recombinant
RabMAb

Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377)

Overview

  • Product name
    Anti-Estrogen Inducible Protein pS2 antibody [EPR3972]
    See all Estrogen Inducible Protein pS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3972] to Estrogen Inducible Protein pS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Estrogen Inducible Protein pS2 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human stomach lysate, MCF-7 cell lysate, Human breast carcinoma tissue, Human stomach tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
  • Tissue specificity
    Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
  • Sequence similarities
    Contains 1 P-type (trefoil) domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCEI antibody
    • Breast cancer estrogen inducible protein antibody
    • Breast cancer estrogen inducible sequence antibody
    • Breast cancer estrogen-inducible protein antibody
    • D21S21 antibody
    • Gastrointestinal trefoil protein antibody
    • Gastrointestinal trefoil protein pS2 antibody
    • hP1.A antibody
    • HP1A antibody
    • HPS 2 antibody
    • HPS2 antibody
    • pNR 2 antibody
    • PNR-2 antibody
    • pNR2 antibody
    • Polypeptide P1.A antibody
    • Protein pS2 antibody
    • PS 2 antibody
    • pS2 antibody
    • pS2 protein antibody
    • TFF 1 antibody
    • TFF1 antibody
    • TFF1_HUMAN antibody
    • Trefoil factor 1 antibody
    see all

Images

  • All lanes : Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 9 kDa

  • ab92377 staining Estrogen Inducible Protein pS2 in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue (A) and Human stomach tissue (B) at 1/100 dilution. Detection: DAB staining.
  • ICC/IF image of ab92377 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92377, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab92377 showing positive staining in Ovarian carcinoma tissue.

  • ab92377 showing negative staining in Normal breast tissue.

  • ab92377 showing negative staining in Normal kidney tissue.

  • ab92377 showing positive staining in Ductal infiltrating breast carcinoma tissue.

  • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Estrogen Inducible Protein pS2 with unpurified ab92377 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Klett H  et al. Identification and Validation of a Diagnostic and Prognostic Multi-Gene Biomarker Panel for Pancreatic Ductal Adenocarcinoma. Front Genet 9:108 (2018). Read more (PubMed: 29675033) »
  • Gonzaga IM  et al. TFF1 hypermethylation and decreased expression in esophageal squamous cell carcinoma and histologically normal tumor surrounding esophageal cells. Clin Epigenetics 9:130 (2017). Read more (PubMed: 29296124) »

See all 7 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 Breast Cancer)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
100000 cells
Specification
MCF7 Breast Cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF-7 Cells)
Specification
MCF-7 Cells
Treatment
1nM Estradiol for 2, 4, 8, and 24 hours
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Marguerite Vantangoli

Verified customer

Submitted Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Pancreas)
Loading amount
200 µg
Specification
Pancreas
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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