Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab92377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionStabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
  • Tissue specificityFound in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
  • Sequence similaritiesContains 1 P-type (trefoil) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCEI antibody
    • Breast cancer estrogen inducible protein antibody
    • Breast cancer estrogen inducible sequence antibody
    • Breast cancer estrogen-inducible protein antibody
    • D21S21 antibody
    • Gastrointestinal trefoil protein antibody
    • Gastrointestinal trefoil protein pS2 antibody
    • hP1.A antibody
    • HP1A antibody
    • HPS 2 antibody
    • HPS2 antibody
    • pNR 2 antibody
    • PNR-2 antibody
    • pNR2 antibody
    • Polypeptide P1.A antibody
    • Protein pS2 antibody
    • PS 2 antibody
    • pS2 antibody
    • pS2 protein antibody
    • TFF 1 antibody
    • TFF1 antibody
    • TFF1_HUMAN antibody
    • Trefoil factor 1 antibody
    see all

Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] images

  • All lanes : Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 9 kDa
  • ab92377 staining Estrogen Inducible Protein pS2 in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue (A) and Human stomach tissue (B) at 1/100 dilution. Detection: DAB staining.
  • ICC/IF image of ab92377 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92377, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab92377 showing positive staining in Ovarian carcinoma tissue.

  • ab92377 showing negative staining in Normal breast tissue.

  • ab92377 showing negative staining in Normal kidney tissue.

  • ab92377 showing positive staining in Ductal infiltrating breast carcinoma tissue.

  • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Estrogen Inducible Protein pS2 with unpurified ab92377 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

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References for Anti-Estrogen Inducible Protein pS2 antibody [EPR3972] (ab92377)

This product has been referenced in:
  • Wu CS  et al. Aberrant JAK/STAT Signaling Suppresses TFF1 and TFF2 through Epigenetic Silencing of GATA6 in Gastric Cancer. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27598141) »
  • Liau JY  et al. Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features. Mod Pathol N/A:N/A (2014). Human . Read more (PubMed: 24406866) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 Breast Cancer)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 100000 cells
Specification MCF7 Breast Cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF-7 Cells)
Specification MCF-7 Cells
Treatment 1nM Estradiol for 2, 4, 8, and 24 hours
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Marguerite Vantangoli

Verified customer

Submitted Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Pancreas)
Loading amount 200 µg
Specification Pancreas
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"