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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Estrogen Receptor alpha.
(Peptide available as ab37519.)
Our Abpromise guarantee covers the use of ab37438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.Can be blocked with Human Estrogen Receptor alpha peptide (ab37519).|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab37438 staining uterus (human and mouse) and testis (rat) tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"