Anti-Estrogen Receptor alpha antibody (ab75635)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Estrogen Receptor alpha antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human, capybara -
Immunogen
Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IP |
Use at 10 µg/mg of lysate.
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IHC-P | (4) |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IP
Use at 10 µg/mg of lysate. |
IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Anti-Estrogen Receptor alpha antibody (ab75635) at 5 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa -
ab75635 at 20 µg/ml dilution staining Estrogen Receptor alpha in breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen.
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Immunohistochemical analysis of capybara testis tissue, labeling Estrogen receptor alpha with ab75635, diluted 1/1000. Heat mediated antigen retrieval with citrate buffer pH 6, blocked with BSA.
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Staining of Estrogen Receptor alpha in MCF7 (Human breast adenocarcinoma cell line cell lysate immunoprecipitated using ab75635 at 10 µg/mg lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab75635 has been referenced in 47 publications.
- Klein-Rodewald T et al. New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance. Sci Rep 12:19793 (2022). PubMed: 36396684
- Fan Q et al. miR-130a-3p promotes cell proliferation and invasion by targeting estrogen receptor a and androgen receptor in cervical cancer. Exp Ther Med 21:414 (2021). PubMed: 33747155
- Gustafsson KL et al. Arginine site 264 in murine estrogen receptor-α is dispensable for the regulation of the skeleton. Am J Physiol Endocrinol Metab 320:E160-E168 (2021). PubMed: 33225718
- Chrzanowski-Smith OJ et al. Resting skeletal muscle PNPLA2 (ATGL) and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences. Exp Physiol 106:1208-1223 (2021). PubMed: 33675111
- Chmielewski-Stivers N et al. Sex-Specific Differences in Toxicity Following Systemic Paclitaxel Treatment and Localized Cardiac Radiotherapy. Cancers (Basel) 13:N/A (2021). PubMed: 34439129