Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595)

Overview

  • Product nameAnti-Estrogen Receptor alpha antibody [SP1], prediluted
    See all Estrogen Receptor alpha primary antibodies
  • Description
    Rabbit monoclonal [SP1] to Estrogen Receptor alpha, prediluted
  • Specificityab27595 recognises estrogen receptor alpha (ER).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide corresponding to Human Estrogen Receptor alpha. Synthetic peptide derived from C-terminal of human estrogen receptor alpha.

  • Positive control
    • Breast carcinomas

Properties

Applications

Our Abpromise guarantee covers the use of ab27595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

USE NEAT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha antibody [SP1], prediluted images

  • Immunohistochemical staining of human breast carcinoma labelling Estrogen Receptor alpha [SP1] with ab93739 at 1:200 dilution.

  • Staining of human breast carcinoma using ab27595
  • Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595) at 1/25 dilution + lysate prepared from MCF7 cellsImage produced using the diluted version of this antibody, ab16660.

References for Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595)

This product has been referenced in:
  • Liu Q  et al. Genetic targeting of sprouting angiogenesis using Apln-CreER. Nat Commun 6:6020 (2015). IHC-Fr . Read more (PubMed: 25597280) »
  • Tian X  et al. Peritruncal coronary endothelial cells contribute to proximal coronary artery stems and their aortic orifices in the mouse heart. PLoS One 8:e80857 (2013). IHC-P ; Mouse . Read more (PubMed: 24278332) »

See all 2 Publications for this product

Product Wall


ab27595 is specific for ER-alpha. We do not have any experimental data regarding the cross-reactivity of ab27595 with ER-beta. But there is no overlap between the immunogen for ab27595 and the ER-beta protein (Q92731), so theoretically it should...

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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Thank you for contacting us.

As antigen retrieval solution, citrate buffer pH 6.0 was used.
Please see below for the detailed protocol:

Pretreatment (Antigen retrieval):

Step 1. Heat slides in citrate buffer in micr...

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Thank you for your inquiry. No, based on immunogen sequence ab27595 will not recognize a sequence between aa 302-553. I am sorry that this information could not be more helpful. Please contact me with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"