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Recombinant fragment corresponding to Human Estrogen Receptor alpha aa 302-595. Recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor alpha.
Database link: P03372
Our Abpromise guarantee covers the use of ab16460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration. Use at a concentration of 2 µg/mg of protein lysate on native and denatured lysate.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).|
|Neutralising||Use at an assay dependent concentration. This antibody can be used to block binding of estrogen to the estrogen receptor.|