Anti-Estrogen Receptor alpha (phospho S104) antibody (ab30656)

Overview

  • Product nameAnti-Estrogen Receptor alpha (phospho S104) antibody
    See all Estrogen Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor alpha (phospho S104)
  • SpecificityThis antibody detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at Serine 104.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Estrogen Receptor alpha around the phosphorylation site of Serine 104.

  • Positive control
    • Extracts of 293 cells treated with forskolin.

Properties

Applications

Our Abpromise guarantee covers the use of ab30656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha (phospho S104) antibody images

  • ab30656 staining human Estrogen Receptor alpha in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.

    Left image antibody un-treated.

    Right image antibody pre-incubated with synthetic phosphopeptide.

  • All lanes : Anti-Estrogen Receptor alpha (phospho S104) antibody (ab30656) at 1 µg/ml

    Lane 1 : 293 cells treated with forskolin. Antibody un-treated.
    Lane 2 : 293 cells treated with forskolin. Antibody pre-incubated with synthetic phosphopeptide.

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa

References for Anti-Estrogen Receptor alpha (phospho S104) antibody (ab30656)

This product has been referenced in:
  • Kalthoff S  et al. Gender matters: estrogen receptor alpha (ERa) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A). J Hepatol 59:797-804 (2013). IHC-P ; Human . Read more (PubMed: 23714156) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. I apologise for the delay in obtaining this information for you. I can confirm that the specificity of antibody (ab30656) has been tested by using a blocking peptide in IHC. Also, when used in ELISA, the reading increase...

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Thank you for your enquiry. The immunogen for this ER alpha antibody is not conserved in ER beta. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"